What is already known:
What this study adds:
The interface separating the upper troposphere from the lower stratosphere is called the tropopause, a complex transition layer contributing to many dynamical aspects of the atmosphere. In this study, observational data is obtained from the ESRL Ozonesonde Data Archive with a multidecadal time-span from 1967-2021 and 1982-2021. Locations in the tropical, polar, and midlatitude regions are examined due to their variation in latitude, climate, and season. After analyzing the tropopause from a composition and stability perspective at each of these areas, the World Meteorological Organization (WMO) lapse-rate definition of 2°C km−1 is evaluated in comparison. Upon investigation, using a 9 K km−1 potential temperature gradient threshold as a stability identifier has proven to be ideal in determining the location of the tropopause in all regions and seasons. The WMO definition and stability threshold are applied to individual profiles and compared to vertical profiles of ozone for evaluation. The stability threshold tends to always mark a tropopause layer directly below the WMO definition in instances where the temperature lapse rate is not met, while the altitude difference between the two vary based on time of year, location and potentially dynamical impacts. Although there are many different definitions that can be used to find the tropopause, the purpose of this study is to investigate the accuracy and consistency of using the stability threshold, especially in situations where the WMO tropopause may not perform well.